Haynes Lab:Notebook/CRISPR Editing/2015/03/11: Difference between revisions

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(Autocreate 2015/03/11 Entry for Haynes_Lab:Notebook/CRISPR_Editing)
 
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| colspan="2"|
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==03//2015==
==03/11/2015==
 
PCR with phusion with primers P197/198<br>Include negative control<br>
 
{| {{table}}
 
| align="center" style="background:#f0f0f0;"|'''Cell type'''
| align="center" style="background:#f0f0f0;"|'''gRNA'''
| align="center" style="background:#f0f0f0;"|'''Rep'''
| align="center" style="background:#f0f0f0;"|'''ul for 200ng'''
|-
| Luc14||g025||1||0.99
|-
| Luc14||g025||2||0.97
|-
| Luc14||g025||3||0.93
|-
| Luc14||g031||1||1.15
|-
| Luc14||g031||2||0.82
|-
| Luc14||g031||3||1.08
|-
| Luc14||g032||1||1.31
|-
| Luc14||g032||2||1.13
|-
| Luc14||g032||3||1.57
|-
| Luc14||g044||1||1.07
|-
| Luc14||g044||2||1.05
|-
| Luc14||g044||3||1.44
|-
| Gal4-EED+dox||g025||1||1.14
|-
| Gal4-EED+dox||g025||2||0.97
|-
| Gal4-EED+dox||g025||3||1.30
|-
| Gal4-EED+dox||g031||1||1.21
|-
| Gal4-EED+dox||g031||2||1.21
|-
| Gal4-EED+dox||g031||3||1.46
|-
| Gal4-EED+dox||g032||1||0.99
|-
| Gal4-EED+dox||g032||2||0.92
|-
| Gal4-EED+dox||g032||3||1.00
|-
| Gal4-EED+dox||g044||1||1.02
|-
| Gal4-EED+dox||g044||2||0.87
|-
| Gal4-EED+dox||g044||3||0.90
|}
<br><br>
Make dilutions
<br><br>
Miniprep KAH228, cut with E and P, run on gel, send for sequencing
<br><br>
Check on cells
<br><br>
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Revision as of 09:09, 11 March 2015

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03/11/2015

PCR with phusion with primers P197/198
Include negative control

Cell type gRNA Rep ul for 200ng
Luc14 g025 1 0.99
Luc14 g025 2 0.97
Luc14 g025 3 0.93
Luc14 g031 1 1.15
Luc14 g031 2 0.82
Luc14 g031 3 1.08
Luc14 g032 1 1.31
Luc14 g032 2 1.13
Luc14 g032 3 1.57
Luc14 g044 1 1.07
Luc14 g044 2 1.05
Luc14 g044 3 1.44
Gal4-EED+dox g025 1 1.14
Gal4-EED+dox g025 2 0.97
Gal4-EED+dox g025 3 1.30
Gal4-EED+dox g031 1 1.21
Gal4-EED+dox g031 2 1.21
Gal4-EED+dox g031 3 1.46
Gal4-EED+dox g032 1 0.99
Gal4-EED+dox g032 2 0.92
Gal4-EED+dox g032 3 1.00
Gal4-EED+dox g044 1 1.02
Gal4-EED+dox g044 2 0.87
Gal4-EED+dox g044 3 0.90



Make dilutions

Miniprep KAH228, cut with E and P, run on gel, send for sequencing

Check on cells


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