Haynes Lab:Notebook/CRISPR Editing/2015/07/08: Difference between revisions
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==07/08/2015== | ==07/08/2015== | ||
Extracted gDNA from Luc14 0.1ug, 0.01ug, Gal4-EED+puro 1.0ug, and Gal4-EED+dox 1.0ug, 0.1ug, and 0.01ug samples. | Extracted gDNA from Luc14 0.1ug, 0.01ug, Gal4-EED+puro 1.0ug, and Gal4-EED+dox 1.0ug, 0.1ug, and 0.01ug samples. | ||
<br> | |||
Ran PCR with P197/198 | |||
:Luc14 | |||
::1.0ug | |||
::0.1ug | |||
::0.01ug, | |||
:Gal4-EED+puro | |||
::1.0ug | |||
:Gal4-EED+dox | |||
::1.0ug | |||
::0.1ug | |||
::0.01ug | |||
<br> | |||
Diluted PCR products 1:1000 fold, ran nested PCR with P163/215<br> | |||
Ran on gel, all looked good, nothing in the negative controls | |||
:no template control from P197/198, negative control template with P163/215 | |||
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Revision as of 09:51, 15 July 2015
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07/08/2015Extracted gDNA from Luc14 0.1ug, 0.01ug, Gal4-EED+puro 1.0ug, and Gal4-EED+dox 1.0ug, 0.1ug, and 0.01ug samples.
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