Haynes Lab:Notebook/CRISPR Editing/2015/08/31: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==08/31/2015== | ||
Surveyor assay on Luc14 dilutions in triplicate, 1ug, 0.1ug, and 0.01ug g034. 1ul surveyor, 1ul enhancer, 1hour at 42deg. after 1h, added 1.9ul of stop solution, diluted 1:20 in water, ran on BA. | |||
<br><br> | |||
Surveyor assay on Luc14 singlet failed gRNAs. 1ul surveyor, 1ul enhancer, 1hour at 42deg. after 1h, added 1.9ul of stop solution, diluted 1:20 in water, ran on BA. | |||
<br><br> | |||
Phusion PCR for sequencing luciferase. template: sheared, untreated, Luc14 gDNA | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''F primer''' | |||
| align="center" style="background:#f0f0f0;"|'''R primer''' | |||
| align="center" style="background:#f0f0f0;"|'''exp size''' | |||
| align="center" style="background:#f0f0f0;"|'''Tann''' | |||
| align="center" style="background:#f0f0f0;"|'''seq primer''' | |||
|- | |||
| P218||P233||1087||61||P218, 283 | |||
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| P161||P233||1799||61||P175 | |||
|- | |||
| P169||P233||1249||61||P171 | |||
|} | |||
PCR purified the samples from the dilution experiment that didn't have enough DNA. | |||
<br><br> | |||
Taq PCR P166/238 sheared untreated Luc14 gDNA, at 47, ext 2:30, length, 2259bp | |||
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Latest revision as of 01:06, 27 September 2017
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08/31/2015Surveyor assay on Luc14 dilutions in triplicate, 1ug, 0.1ug, and 0.01ug g034. 1ul surveyor, 1ul enhancer, 1hour at 42deg. after 1h, added 1.9ul of stop solution, diluted 1:20 in water, ran on BA.
Surveyor assay on Luc14 singlet failed gRNAs. 1ul surveyor, 1ul enhancer, 1hour at 42deg. after 1h, added 1.9ul of stop solution, diluted 1:20 in water, ran on BA.
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