Haynes Lab:Notebook/CRISPR Editing/2015/08/31

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08/31/2015

Surveyor assay on Luc14 dilutions in triplicate, 1ug, 0.1ug, and 0.01ug g034. 1ul surveyor, 1ul enhancer, 1hour at 42deg. after 1h, added 1.9ul of stop solution, diluted 1:20 in water, ran on BA.

Surveyor assay on Luc14 singlet failed gRNAs. 1ul surveyor, 1ul enhancer, 1hour at 42deg. after 1h, added 1.9ul of stop solution, diluted 1:20 in water, ran on BA.



Phusion PCR for sequencing luciferase. template: sheared, untreated, Luc14 gDNA

F primer R primer exp size Tann seq primer
P218P233108761P218, 283
P161P233179961P175
P169P233124961P171


PCR purified the samples from the dilution experiment that didn't have enough DNA.

Taq PCR P166/238 sheared untreated Luc14 gDNA, at 47, ext 2:30, length, 2259bp


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