Haynes Lab:Notebook/CRISPR Editing/2015/08/31: Difference between revisions
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Surveyor assay on Luc14 dilutions in triplicate, 1ug, 0.1ug, and 0.01ug g034. 1ul surveyor, 1ul enhancer, 1hour at 42deg. after 1h, added 1.9ul of stop solution, diluted 1:20 in water, ran on BA. | Surveyor assay on Luc14 dilutions in triplicate, 1ug, 0.1ug, and 0.01ug g034. 1ul surveyor, 1ul enhancer, 1hour at 42deg. after 1h, added 1.9ul of stop solution, diluted 1:20 in water, ran on BA. | ||
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Surveyor assay on Luc14 singlet failed gRNAs. 1ul surveyor, 1ul enhancer, 1hour at 42deg. after 1h, added 1.9ul of stop solution, diluted 1:20 in water, ran on BA. | |||
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Phusion PCR for sequencing luciferase. template: sheared, untreated, Luc14 gDNA | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''F primer''' | |||
| align="center" style="background:#f0f0f0;"|'''R primer''' | |||
| align="center" style="background:#f0f0f0;"|'''exp size''' | |||
| align="center" style="background:#f0f0f0;"|'''Tann''' | |||
| align="center" style="background:#f0f0f0;"|'''seq primer''' | |||
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| P218||P233||1087||61||P218, 283 | |||
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| P161||P233||1799||61||P175 | |||
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| P169||P233||1249||61||P171 | |||
|} | |||
PCR purified the samples from the dilution experiment that didn't have enough DNA. | PCR purified the samples from the dilution experiment that didn't have enough DNA. | ||
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Revision as of 13:25, 31 August 2015
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08/31/2015Surveyor assay on Luc14 dilutions in triplicate, 1ug, 0.1ug, and 0.01ug g034. 1ul surveyor, 1ul enhancer, 1hour at 42deg. after 1h, added 1.9ul of stop solution, diluted 1:20 in water, ran on BA.
Surveyor assay on Luc14 singlet failed gRNAs. 1ul surveyor, 1ul enhancer, 1hour at 42deg. after 1h, added 1.9ul of stop solution, diluted 1:20 in water, ran on BA.
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