Haynes Lab:Notebook/CRISPR Editing/2015/10/11: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==10/11/2015== | ||
Surveyor of g055 and test of g054, 42deg for 1h, 1.9ul stop solution, dilute 1:20, run on BA | |||
<br><br> | |||
Set up phusion PCR of sheared Luc14 gDNA with | |||
:P160/238 | |||
:P161/233 | |||
:P237/239 | |||
<br> | |||
annealing temp 60, ext time 2min | |||
<br><br> | |||
changed media on transfected cells to media with antibiotics (including dox or puro) | |||
Latest revision as of 01:14, 27 September 2017
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10/11/2015Surveyor of g055 and test of g054, 42deg for 1h, 1.9ul stop solution, dilute 1:20, run on BA
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