Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/02/28: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Rene M Davis (talk | contribs) (Autocreate 2013/02/28 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs) |
Rene M Davis (talk | contribs) |
||
(One intermediate revision by the same user not shown) | |||
Line 7: | Line 7: | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
==Entry title== | ==Entry title== | ||
Did minipreps on the colonies picked 2013-02-27 (shook for 24 hours)<br> | |||
Many of them did not work. This chart shows which ones worked with an X and which ones did not grow with a 0: | |||
{| {{table}} | |||
|- | |||
| Part number || Description || Part size (bp) || Backbone || Colonies | |||
|- | |||
| || || || || 1 || 2 | |||
|- | |||
| F2620 || p(tetR)-RBS-luxR-Stop-lux pR || 1061 || pSB1A2 || 0 || 0 | |||
|- | |||
| K082035 || p(tetR)-RBS-rhlI-Stop-Stop || 884 || pSB1A2 || X || X | |||
|- | |||
| R0071 || RhlR-activated promoter || 53 || pSB1A2 || 0 || X | |||
|- | |||
| K084007 || LacI-RBS-LasI || 835 || pSB1AK3 || X || X | |||
|- | |||
| K081016 || RBS-lasI-Stop || 735 || pSB1AK3 || 0 || X | |||
|- | |||
| K081023 || RBS-cl lam-RBS-lasR-Stop || 1653 || pSB1AK3 || X || 0 | |||
|} | |||
<br> | |||
Picked more colonies and grew them in 3ml LB + amp | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | ||
|} | |} | ||
__NOTOC__ | __NOTOC__ |
Revision as of 13:52, 4 March 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||
Entry titleDid minipreps on the colonies picked 2013-02-27 (shook for 24 hours)
Picked more colonies and grew them in 3ml LB + amp |