Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/03/01

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Miniprepped the colonies grown up yesterday

Part number Description Part size (bp) Backbone Colonies
1 2 3 4 5
F2620 p(tetR)-RBS-luxR-Stop-lux pR 1061 pSB1A2 0 0 X X 0
K082035 p(tetR)-RBS-rhlI-Stop-Stop 884 pSB1A2 X X
R0071 RhlR-activated promoter 53 pSB1A2 X
K084007 LacI-RBS-LasI 835 pSB1AK3 X X
K081016 RBS-lasI-Stop 735 pSB1AK3 0 X
K081023 RBS-cl lam-RBS-lasR-Stop 1653 pSB1AK3 X 0 X

Also tried a second elution on the miniprep columns using 50ul water. Got pretty decent yields from that but need to confirm on a gel that it's a good idea.


Pulled more parts out of the registry

Part number Description Part size (bp) Backbone
K145254 p(tetR)-RBS-luxR-Stop-lux pR 1061 pSB1A2
K082035 p(tetR)-RBS-rhlI-Stop-Stop 884 pSB1A2
R0071 RhlR-activated promoter 53 pSB1A2
K084007 LacI-RBS-LasI 835 pSB1AK3
K081016 RBS-lasI-Stop 735 pSB1AK3
K081023 RBS-cl lam-RBS-lasR-Stop 1653 pSB1AK3


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