Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/02/10: Difference between revisions
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Rene M Davis (talk | contribs) (Autocreate 2014/02/10 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs) |
Rene M Davis (talk | contribs) |
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| colspan="2"| | | colspan="2"| | ||
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== | ==02/10/2014== | ||
* | Ran gel of PCR products from 2/8/14. <br> | ||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Sample''' | |||
| align="center" style="background:#f0f0f0;"|'''Primer 1''' | |||
| align="center" style="background:#f0f0f0;"|'''Primer 2''' | |||
| align="center" style="background:#f0f0f0;"|'''Template''' | |||
| align="center" style="background:#f0f0f0;"|'''Ideal AT''' | |||
| align="center" style="background:#f0f0f0;"|'''Approx Length''' | |||
|- | |||
| 1||P098||P099||I13522 (2)||46||2372 | |||
|- | |||
| 2||P098||P099||I13522 (3)||46||2372 | |||
|- | |||
| 3||P098||P099||I13522 9.27 55.7||46||2372 | |||
|- | |||
| 4||P103||P104||I13522 (2)||49||780 | |||
|- | |||
| 5||P103||P104||I13522 (3)||49||780 | |||
|- | |||
| 6||P103||P104||I13522 9.27 55.7||49||780 | |||
|} | |||
<br> | |||
***insert gel photo before and after*** | |||
<br> | |||
Gel extract using Zymo kit. Read concentrations of these and gel extractions from 2/7/14: | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Primer 1''' | |||
| align="center" style="background:#f0f0f0;"|'''Primer 2''' | |||
| align="center" style="background:#f0f0f0;"|'''Template''' | |||
| align="center" style="background:#f0f0f0;"|'''260''' | |||
| align="center" style="background:#f0f0f0;"|'''280''' | |||
| align="center" style="background:#f0f0f0;"|'''260/280''' | |||
| align="center" style="background:#f0f0f0;"|'''ng/µL''' | |||
|- | |||
| ||||water||0.003||0.002||1.933||3.084 | |||
|- | |||
| P103||P104||I13522 (2)||0.124||0.065||1.9||124.369 | |||
|- | |||
| P103||P104||I13522 (3)||0.123||0.065||1.9||123.324 | |||
|- | |||
| P103||P104||I13522 9/27/13 55.7||0.054||0.028||1.918||54.117 | |||
|- | |||
| P098||P099||I13522 (3)||0.098||0.051||1.931||97.574 | |||
|- | |||
| P098||P099||I13522 (2)||0.116||0.06||1.943||115.692 | |||
|- | |||
| P117||P118||K092900 (RhlR)||0.05||0.027||1.874||50.159 | |||
|- | |||
| P105||P106||R0011 (2)||0.005||0.002||2.632||5.229 | |||
|- | |||
| P113||P114||LuxR (F2620)||0.057||0.029||1.968||57.356 | |||
|- | |||
| P105||P106||R0011 (1)||0.002||0||-6.667||2.073 | |||
|- | |||
| P088||P102||Receiver vector||0.113||0.06||1.896||113.028 | |||
|- | |||
| | |||
|} | |||
Golden gate to build LuxR receiver | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Part''' | |||
| align="center" style="background:#f0f0f0;"|'''Length (bp)''' | |||
| align="center" style="background:#f0f0f0;"|'''Concentration (ng/ul)''' | |||
| align="center" style="background:#f0f0f0;"|'''fmol/ul''' | |||
| align="center" style="background:#f0f0f0;"|'''Final volume to dilute 1ul to get 20 fmol/ul''' | |||
|- | |||
| Receiver vector||2372||113||72.2||3.6 | |||
|- | |||
| Lux box||56||20||541.1||27.1 | |||
|- | |||
| -35-B0033-GFP-TT||945||124||198.8||9.9 | |||
|- | |||
| R0011-RBS||85||5||89.1||4.5 | |||
|- | |||
| LuxR||817||57||105.7||5.3 | |||
|} | |||
GG to build RhlR receiver | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Part''' | |||
| align="center" style="background:#f0f0f0;"|'''Length (bp)''' | |||
| align="center" style="background:#f0f0f0;"|'''Concentration (ng/ul)''' | |||
| align="center" style="background:#f0f0f0;"|'''fmol/ul''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
|- | |||
| Receiver vector||2372||113||72.2||3.6 | |||
|- | |||
| Rhl box||56||20||541.1||27.1 | |||
|- | |||
| -35-B0033-GFP-TT||945||124||198.8||9.9 | |||
|- | |||
| R0011-RBS||85||5||89.1||4.5 | |||
|- | |||
| RhlR||765||50||99.0||5.0 | |||
|} | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Revision as of 16:14, 10 February 2014
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
02/10/2014Ran gel of PCR products from 2/8/14.
Golden gate to build LuxR receiver
GG to build RhlR receiver
|