Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/02/10

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(mm/dd/yyyy)
(02/10/2014)
Line 30: Line 30:
<br>
<br>
***insert gel photo before and after***
***insert gel photo before and after***
-
 
+
<br>
 +
Gel extract using Zymo kit. Read concentrations of these and gel extractions from 2/7/14:
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Primer 1'''
 +
| align="center" style="background:#f0f0f0;"|'''Primer 2'''
 +
| align="center" style="background:#f0f0f0;"|'''Template'''
 +
| align="center" style="background:#f0f0f0;"|'''260'''
 +
| align="center" style="background:#f0f0f0;"|'''280'''
 +
| align="center" style="background:#f0f0f0;"|'''260/280'''
 +
| align="center" style="background:#f0f0f0;"|'''ng/µL'''
 +
|-
 +
| ||||water||0.003||0.002||1.933||3.084
 +
|-
 +
| P103||P104||I13522 (2)||0.124||0.065||1.9||124.369
 +
|-
 +
| P103||P104||I13522 (3)||0.123||0.065||1.9||123.324
 +
|-
 +
| P103||P104||I13522 9/27/13 55.7||0.054||0.028||1.918||54.117
 +
|-
 +
| P098||P099||I13522 (3)||0.098||0.051||1.931||97.574
 +
|-
 +
| P098||P099||I13522 (2)||0.116||0.06||1.943||115.692
 +
|-
 +
| P117||P118||K092900 (RhlR)||0.05||0.027||1.874||50.159
 +
|-
 +
| P105||P106||R0011 (2)||0.005||0.002||2.632||5.229
 +
|-
 +
| P113||P114||LuxR (F2620)||0.057||0.029||1.968||57.356
 +
|-
 +
| P105||P106||R0011 (1)||0.002||0||-6.667||2.073
 +
|-
 +
| P088||P102||Receiver vector||0.113||0.06||1.896||113.028
 +
|-
 +
|
 +
|}

Revision as of 18:12, 10 February 2014

Project name Main project page
Previous entry      Next entry

02/10/2014

Ran gel of PCR products from 2/8/14.

Sample Primer 1 Primer 2 Template Ideal AT Approx Length
1P098P099I13522 (2)462372
2P098P099I13522 (3)462372
3P098P099I13522 9.27 55.7462372
4P103P104I13522 (2)49780
5P103P104I13522 (3)49780
6P103P104I13522 9.27 55.749780


      • insert gel photo before and after***


Gel extract using Zymo kit. Read concentrations of these and gel extractions from 2/7/14:

Primer 1 Primer 2 Template 260 280 260/280 ng/µL
water0.0030.0021.9333.084
P103P104I13522 (2)0.1240.0651.9124.369
P103P104I13522 (3)0.1230.0651.9123.324
P103P104I13522 9/27/13 55.70.0540.0281.91854.117
P098P099I13522 (3)0.0980.0511.93197.574
P098P099I13522 (2)0.1160.061.943115.692
P117P118K092900 (RhlR)0.050.0271.87450.159
P105P106R0011 (2)0.0050.0022.6325.229
P113P114LuxR (F2620)0.0570.0291.96857.356
P105P106R0011 (1)0.0020-6.6672.073
P088P102Receiver vector0.1130.061.896113.028



Personal tools