Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/15

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Receiver golden gate with newly prepped receiver intermediate vector

Part ng/µL fmol/ul ml for 200fmol
Receiver int vec56.995277.407407407

Reaction Rec Bb pLux-GFP pLac-LuxR BsmBI T4 Ligase T4 Ligase buffer H2O
Bb ctrl7.4000.51.2528.85
Lux Rec7.42.942.250.51.2523.66
Rhl Rec7.42.781.680.51.2524.39
Esa Rec7.44.352.70.51.2521.8

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