Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/05/01: Difference between revisions
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PCR Optimization of RhlI. 4 PCR tubes with DMSO, 4 PCR tubes without DMSO. <br><br> | PCR Optimization of RhlI. 4 PCR tubes with DMSO, 4 PCR tubes without DMSO. Tubes were placed in the PCR machine with a temperature gradient. (range?)<br><br> | ||
PCR-purified synthase PCR products. Next, they are ready to be digested and then ligated with the backbone. <br> | PCR-purified synthase PCR products. Next, they are ready to be digested and then ligated with the backbone. <br> | ||
Revision as of 19:04, 28 May 2015
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05/1/2015
PCR Optimization of RhlI. 4 PCR tubes with DMSO, 4 PCR tubes without DMSO. Tubes were placed in the PCR machine with a temperature gradient. (range?) PCR-purified synthase PCR products. Next, they are ready to be digested and then ligated with the backbone.
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