Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/06/17: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==06/17/2015== | ==06/17/2015== | ||
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Digest verification for senders that I mini-prepped yesterday looks promising: [[Image:15.06.19 senders digest verification.JPG copy.jpg | 800px]] | Digest verification for senders that I mini-prepped yesterday looks promising: [[Image:15.06.19 senders digest verification.JPG copy.jpg | 800px]] <br> | ||
Two bands for CerI were expected since there are two EcoRI sites in the synthase. EsaI 2 and 3 looked like they were successful; these bands are longer than the others—since part of EsaI appeared to be truncated last time, the shorter bands probably represent the truncated plasmid. <br> | |||
I will send the following plasmids out for sequencing: <br> | |||
EsaI 2, EsaI 3, CerI 2, LasI 3, BjaI 3, BjaI 4 | |||
Latest revision as of 01:01, 27 September 2017
Project name | Main project page Previous entry Next entry |
06/17/2015
Digest verification for senders that I mini-prepped yesterday looks promising: Two bands for CerI were expected since there are two EcoRI sites in the synthase. EsaI 2 and 3 looked like they were successful; these bands are longer than the others—since part of EsaI appeared to be truncated last time, the shorter bands probably represent the truncated plasmid.
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