Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/06/17: Difference between revisions
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==06//2015== | ==06/17/2015== | ||
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Digest verification for senders that I mini-prepped yesterday looks promising: [[Image:15.06.19 senders digest verification.JPG copy.jpg | 800px]] <br> | |||
Two bands for CerI were expected since there are two EcoRI sites in the synthase. EsaI 2 and 3 looked like they were successful; these bands are longer than the others—since part of EsaI appeared to be truncated last time, the shorter bands probably represent the truncated plasmid. <br> | |||
I will send the following plasmids out for sequencing: <br> | |||
EsaI 2, EsaI 3, CerI 2, LasI 3, BjaI 3, BjaI 4 | |||
Revision as of 19:21, 25 June 2015
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06/17/2015
Digest verification for senders that I mini-prepped yesterday looks promising: Two bands for CerI were expected since there are two EcoRI sites in the synthase. EsaI 2 and 3 looked like they were successful; these bands are longer than the others—since part of EsaI appeared to be truncated last time, the shorter bands probably represent the truncated plasmid.
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