Haynes Lab:Notebook/David Barclay Undergrad Training/Plasmid Transformation/2013/10/03: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Haynes BioBrick Cloning</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Haynes BioBrick Cloning</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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=Continuation of Restriction Digests and Gel Electrophoresis= | |||
Restriction Digest Table<br> | |||
* Checked plasmid minipreps with EcoRI/PstI digests | |||
{| {{table}} border="1" cellspacing="3" | |||
<!-- Editing: the coding for this table is a bit more advanced. --> | |||
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<!-- The next two "rowspan=7" cells span all 7 rows in the table so that they can fit the "Expected" list and a gel image. rowspan is how you create merged rows in Wiki code. --> | |||
<!-- Replace Plasmid 1 and 2 with the names of your plasmids. Replace insert size and vector size with appropriate information for your plasmids. If you only have one Plasmid, delete all the text for Plasmid 2 | |||
<!-- After you upload your gel image to OWW, replace "GelImage.jpg" with the name of your image file --> | |||
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|- valign="top" | |||
| bgcolor=#cfcfcf | Reagent | |||
| bgcolor=#cfcfcf | Volume | |||
| rowspan="7" | <u>Expected:</u><br>1. fshPCD = 186 bp, 4968 bp<br> | |||
| rowspan="7" | [[Image:Gel DKB 10-3-13.jpg|400px|Today's gel]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | |||
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| DNA(plasmid) || 5.0 μL | |||
|- | |||
| 10X FD Buffer || 1.5 | |||
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| EcoRI || 1.0 | |||
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| PstI || 1.0 | |||
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| dH<sub>2</sub>O || 6.5 | |||
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| || 15 μL --> 37°C/ 15 min. | |||
|} | |||
'''Conclusion''' | |||
There were faint bands at the 186 position; however, they are harder to see on the picture. The 4968 bands for all 3 are very visible. Fairly successful digest. | |||
'''DNA Sequencing Samples''' | |||
<!-- * signs create an automatically bulleted list. Replace 'date' with the date you submitted the samples --> | |||
<!-- The Order Number is shown at the top of your sequencing order form. Record in the indicated spot below --> | |||
* Submitted to DNASU on: 10/7/2013 | |||
* Order Number: 8213 | |||
<!-- # signs create an automatically numbered list. Replace Plasmid 1 & 2 with the names of your plasmids. Replace 'name' with the appropriate name of each primer. --> | |||
# Plasmid 1 (fshPCD) - Forward P001 | |||
# Plasmid 1 (fshPCD) - Reverse P002 | |||
Latest revision as of 23:24, 26 September 2017
Haynes BioBrick Cloning | Main project page Previous entry Next entry | ||||||||||||||||
Continuation of Restriction Digests and Gel ElectrophoresisRestriction Digest Table
Conclusion There were faint bands at the 186 position; however, they are harder to see on the picture. The 4968 bands for all 3 are very visible. Fairly successful digest. DNA Sequencing Samples
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