Haynes Lab:Notebook/David Barclay Undergrad Training/Plasmid Transformation/2013/10/03: Difference between revisions
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(Autocreate 2013/10/03 Entry for Haynes_Lab:Notebook/David_Barclay_Undergrad_Training/Plasmid_Transformation) |
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=Continuation of Restriction Digests and Gel Electrophoresis= | |||
Restriction Digest Table<br> | |||
* Checked plasmid minipreps with EcoRI/PstI digests | |||
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<!-- Replace Plasmid 1 and 2 with the names of your plasmids. Replace insert size and vector size with appropriate information for your plasmids. If you only have one Plasmid, delete all the text for Plasmid 2 | |||
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|- valign="top" | |||
| bgcolor=#cfcfcf | Reagent | |||
| bgcolor=#cfcfcf | Volume | |||
| rowspan="7" | <u>Expected:</u><br>1. fshPCD = 186 bp, 4968 bp<br> | |||
| rowspan="7" | [[Image:Gel DKB 10-3-13.jpg|400px|Today's gel]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | |||
|- | |||
| DNA(plasmid) || 5.0 μL | |||
|- | |||
| 10X FD Buffer || 1.5 | |||
|- | |||
| EcoRI || 1.0 | |||
|- | |||
| PstI || 1.0 | |||
|- | |||
| dH<sub>2</sub>O || 6.5 | |||
|- | |||
| || 15 μL --> 37°C/ 15 min. | |||
|} | |||
'''Conclusion''' | |||
There were faint bands at the 186 position; however, they are harder to see on the picture. The 4968 bands for all 3 are very visible. Fairly successful digest. | |||
'''DNA Sequencing Samples''' | |||
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* Submitted to DNASU on: 10/7/2013 | |||
* Order Number: 8213 | |||
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# Plasmid 1 (fshPCD) - Forward P001 | |||
# Plasmid 1 (fshPCD) - Reverse P002 | |||
Revision as of 14:17, 7 October 2013
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Continuation of Restriction Digests and Gel ElectrophoresisRestriction Digest Table
Conclusion There were faint bands at the 186 position; however, they are harder to see on the picture. The 4968 bands for all 3 are very visible. Fairly successful digest. DNA Sequencing Samples
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