Haynes Lab:Notebook/David Barclay Undergrad Training/Plasmid Transformation/2013/10/03

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Continuation of Restriction Digests and Gel Electrophoresis

Restriction Digest Table

  • Checked plasmid minipreps with EcoRI/PstI digests
Reagent Volume Expected:
1. hPCD = 186 bp, 4968 bp
2. Ubc = 368 bp, 4968 bp
Today's gel
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 5.0 μL
10X FD Buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 6.5
  15 μL --> 37°C/ 15 min.

Conclusion There were definite gel lines at ~4900 and faint lines at ~200 for hPCD (lanes 1,2,5) and ~ 350 for Ubc (3,4,6).
This signifies a working gel.

DNA Sequencing Samples

  • Submitted to DNASU on: '8/14/13'
  • Order Number: 8043
  1. Plasmid 1 (hPCD) - Forward P001
  2. Plasmid 1 (hPCD) - Reverse P002
  3. Plasmid 4 (Ubc) - Forward P001
  4. Plasmid 4 (Ubc) - Reverse P002



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