Haynes Lab:Notebook/David Barclay Undergrad Training/Plasmid Transformation/2015/08/04: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Haynes BioBrick Cloning</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Haynes BioBrick Cloning</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Plasmid Extraction and Validation==
==Plasmid Extraction and Validation==
*B0004 MV2
*BD004 MV2
*B0006 MV2
*BD006 MV2
*Culture inoculation 8-4-15 (19 hour incubation)
*Culture inoculation 8-4-15 (19 hour incubation)
**Culture 1-4: growth
**Culture 1-4: growth
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**Streak plate Colony 1: growth
**Streak plate Colony 1: growth
**Streak plate colony 2: growth
**Streak plate colony 2: growth
*B0005 MV2
*BD006 MV2
**Streak plate colony 1: growth
**Streak plate colony 1: growth
**Streak plate colony 2: growth
**Streak plate colony 2: growth

Latest revision as of 01:04, 27 September 2017

Haynes BioBrick Cloning Main project page
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Plasmid Extraction and Validation

  • BD004 MV2
  • BD006 MV2
  • Culture inoculation 8-4-15 (19 hour incubation)
    • Culture 1-4: growth
    • Culture 2-4: growth
    • Culture 1-6: growth
    • Culture 2-6: growth
  • B0004 MV2
    • Streak plate Colony 1: growth
    • Streak plate colony 2: growth
  • BD006 MV2
    • Streak plate colony 1: growth
    • Streak plate colony 2: growth
  • Sample 1-4= B0004 MV2 colony 1
    • OD260/280=1.90
    • Concentration: 229.7 ng/μL
  • Sample 2-4 = B0004 MV2 colony 2
    • OD260/280=1.99
    • Concentration: 125.05 ng/μL
  • Sample 1-6 = B0006 MV2 colony 1
    • OD260/280= 1.957
    • Concentration: 160.15 ng/μL
  • Sample 2-6= B0006 MV2 colony 2
    • OD260/280 = 2.103
    • Concentration: 35.733 ng/μL
  1. used 50 μL H2O for DNA elution


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