Haynes Lab:Notebook/Engineering PC-TFs/2012/02/16: Difference between revisions
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== | ==Ligation and Assembly== | ||
* | * Weight gel slices. | ||
* Add three times amount of volume to each tube of gel (600 µL) | |||
* Incubate at 55°C for 5-10 minutes. | |||
* Centrifuge for a minute at 17,000 x g. | |||
* Add 20 µL of wash buffer. | |||
* Don't dump supernatent and centrifuge for one minute again. | |||
* Place spin columns in 1.5 mL tubes. | |||
Measure concentration of each fragment: | |||
* Load Gen 5 2.0 software. Go to the Task Manager-Take 3 Application | |||
* Go to Nucleic Acid Quantification. | |||
* Pipette 2 µL of each DNA sequence into Take 3 plate. | |||
* Place plate in EPOCH plate reader. | |||
Revision as of 19:00, 17 February 2012
 Engineering PC-TFs
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Ligation and Assembly
Measure concentration of each fragment:
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