Haynes Lab:Notebook/Engineering PC-TFs/2012/02/16: Difference between revisions

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Results:
Results:


KAH01    96.477    ng/µL
*KAH01    96.477    ng/µL
 
*KAH03    122.149  ng/µL
----
*KAH04    80.182    ng/µL
 
*KAH204  27.054    ng/µL
KAH03    122.149  ng/µL
*KAH205  47.105    ng/µL
KAH04    80.182    ng/µL
*KAH206  45.217    ng/µL
KAH204  27.054    ng/µL
*KAH225  66.047    ng/µL
KAH205  47.105    ng/µL
KAH206  45.217    ng/µL
KAH225  66.047    ng/µL


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Revision as of 19:11, 17 February 2012

Engineering PC-TFs <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Ligation/Assembly

  • Weight gel slices.
  • Add three times amount of volume to each tube of gel (600 µL)
  • Incubate at 55°C for 5-10 minutes.
  • Centrifuge for a minute at 17,000 x g.
  • Add 20 µL of wash buffer.
  • Don't dump supernatent and centrifuge for one minute again.
  • Place spin columns in 1.5 mL tubes.

Measure concentration of each fragment:

  • Load Gen 5 2.0 software. Go to the Task Manager-Take 3 Application
  • Go to Nucleic Acid Quantification.
  • Pipette 2 µL of each DNA sequence into Take 3 plate.
  • Place plate in EPOCH plate reader.

Results:

  • KAH01 96.477 ng/µL
  • KAH03 122.149 ng/µL
  • KAH04 80.182 ng/µL
  • KAH204 27.054 ng/µL
  • KAH205 47.105 ng/µL
  • KAH206 45.217 ng/µL
  • KAH225 66.047 ng/µL


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