Haynes Lab:Notebook/Engineering PC-TFs/2013/03/18: Difference between revisions
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*Enzyme 2 1.0 µL | *Enzyme 2 1.0 µL | ||
*10x buffer 3.0 µL | *10x buffer 3.0 µL | ||
*dH2O 5.0 uL | *dH2O 5.0 uL | ||
* = 30.0 uL </font> | * = 30.0 uL </font> | ||
|} | |} |
Revision as of 20:34, 22 March 2013
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3/18/22Cutting inserts to be placed in mammalian vector A) Get biobricks and enzymes from freezer. PstI, XbaI The following list of parts are:
Digest Order: Biobrick Restriction Enzymes
Follow steps for gel electrophoresis.
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