Haynes Lab:Notebook/Engineering PC-TFs/2013/09/26: Difference between revisions
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| PstI|| 1.0 μL | | PstI|| 1.0 μL | ||
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| Green 10x FastDigest buffer || | | Green 10x FastDigest buffer || 0μL | ||
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| dH<sub>2</sub>O || | | dH<sub>2</sub>O || 18 μL | ||
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| || 30 μL --> 37°C/ ~10 min. | | || 30 μL --> 37°C/ ~10 min. | ||
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[[Image:hiddendragonsofthetemple.jpg|250px|CMV PEG/MgCl<sub>2</sub> Precipitation 9/26/2013]] | [[Image:hiddendragonsofthetemple.jpg|250px|CMV PEG/MgCl<sub>2</sub> Precipitation 9/26/2013]] | ||
Higher molecular mass DNA precipitates at lower PEG concentrations than lower molecular mass DNA, according to [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC342844/ Lis and Schleif 1975, NAR]. I kept DNA from the supernatant and discarded the precipitated DNA. | |||
Therefore, I should see larger bands at 2% PEG and smaller bands at 15% PEG. | |||
Latest revision as of 23:23, 26 September 2017
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September 26, 2013Restriction Digest
PEG/MgCl2 Precipitation
DNA Clean & Concentrator
Gel Electrophoresis
Higher molecular mass DNA precipitates at lower PEG concentrations than lower molecular mass DNA, according to Lis and Schleif 1975, NAR. I kept DNA from the supernatant and discarded the precipitated DNA. Therefore, I should see larger bands at 2% PEG and smaller bands at 15% PEG.
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