Haynes Lab:Notebook/Engineering PC-TFs/2013/10/20: Difference between revisions
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== | ==October 20, 2013== | ||
* | ''Grow liquid culture of CMV + MV9, KAH126'' | ||
*Label 15 ml sterile culture tubes. Fill each tube with 2 ml LB growth medium with Amp. | |||
*Use sterile pipette tip to touch bacterial streak and put the tip into LB medium. | |||
*Grow the cultures for 10-12 hrs. | |||
[[Image:supercali.jpg|200px|Bacterial Transformation of KAH126 and Ligation of CMV + MV9]] | |||
*<span style="font-size:95%"> Bacterial transformation of KAH126 was successful. Restriction digest will need to be to confirm successful ligation. Additionally, presence of film showed on control. Reason for contamination is unknown. | |||
Revision as of 14:33, 20 October 2013
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October 20, 2013Grow liquid culture of CMV + MV9, KAH126
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