Haynes Lab:Notebook/Engineering PC-TFs/2014/04/08: Difference between revisions

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{| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table -->
{| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table -->
|- valign="top"
|- valign="top"
| rowspan="7" | [[Image:2014-04-10 15.47.jpg|400px|Hover name]]<br>15 μL/lane; 1% agarose;  
| rowspan="7" | [[Image:2014-04-10 15.47.jpg|400px|Hover name]]<br>15 μL/lane; 1% agarose; band are at ~ 645bp (both lanes are the same product).
  [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
  [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
|}
|}

Revision as of 18:14, 18 April 2014



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Summary

  • PCR Reaction
Reagent Volume Expected:
1. PCR Reaction Product = 845bp
DNA(CMV/MV9) 0.5 μL
2X GoTaq 12.5 μL
FP DD122 B 1.0 μL
RP DD123 B 1.0 μL
dH2O 10.0 μL
  • Placed reaction mixture into PCR machine on setting GOTAQ35cyc
  • Gel verification
Hover name
15 μL/lane; 1% agarose; band are at ~ 645bp (both lanes are the same product). 
Ladder
  • Expected base pair length: 643




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