Summary
Digest All the Things Addendum
Well One: Ladder
Well Two
Reagent
|
Volume
|
Expected: 2520(insert)+3265(V0120 Vector) = 5785bp Observed: ~7000bp
|
DNA(BL05 M.P) |
8.0 μL
|
10X buffer |
1.5 μL
|
XbaI |
1.0 μL
|
dH2O |
4.5 μL
|
Total |
15 μL --> 37°C/ 30 min.
|
Well Three
Reagent
|
Volume
|
Expected: 2520(insert)+3265(V0120 Vector) = 5785bp Observed: ~7000bp
|
DNA(BL05A) |
4.0 μL
|
10X buffer |
1.5 μL
|
XbaI |
1.0 μL
|
dH2O |
8.5 μL
|
Total |
15 μL --> 37°C/ 30 min.
|
Well Four
Reagent
|
Volume
|
Expected: 1431 (insert) + 3265 (V0120 Vector) = 4696bp Observed: ~4500
|
DNA(BL09 M.P.) |
8.0 μL
|
10X buffer |
1.5 μL
|
XbaI |
1.0 μL
|
dH2O |
4.5 μL
|
Total |
15 μL --> 37°C/ 30 min.
|
Well Five
Reagent
|
Volume
|
Expected: 1431 (insert) + 3265 (V0120 Vector) = 4696bp Observed: ~4000
|
DNA(BL09B) |
4.0 μL
|
10X buffer |
1.5 μL
|
XbaI |
1.0 μL
|
dH2O |
8.5 μL
|
Total |
15 μL --> 37°C/ 30 min.
|
Well Six
Reagent
|
Volume
|
Expected: 5197bp Observed: ~4900
|
DNA(Phosphorylated CMV/MV9 ) |
2.0 μL
|
10X buffer |
1.5 μL
|
dH2O |
11.5 μL
|
Total |
15 μL --> 37°C/ 30 min.
|
Gel Verification
|