Haynes Lab:Notebook/Engineering PC-TFs/2014/06/27

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Summary

Gel Verification

Digest All the Things

Well One: Ladder
Well Two & Three

Reagent Volume Expected:
2559bp, 3226bp
Observed:
~3500bp, ~2800bp
DNA(BL01 C1, C2) 2.0 μL
10X buffer 1.5 μL
PstI 1.0 μL
EcorI 1.0 μL
dH2O 9.5 μL
Total 15 μL --> 37°C/ 30 min.


Well Five & Six

Reagent Volume Expected:
2559bp, 3226bp
Observed:
~3500bp, ~2800bp
DNA(BL05 C1, C2) 2.0 μL
10X buffer 1.5 μL
PstI 1.0 μL
EcorI 1.0 μL
dH2O 9.5 μL
Total 15 μL --> 37°C/ 30 min.

Well Four & Seven

Reagent Volume Expected:
1482bp, 3226bp
Observed:
~3500bp, ~1500bp
DNA(BL09 C1, C2) 2.0 μL
10X buffer 1.5 μL
PstI 1.0 μL
EcorI 1.0 μL
dH2O 9.5 μL
Total 15 μL --> 37°C/ 30 min.


Well Eight & Nine

Reagent Volume Expected:
4552bp, 653bp
Observed:
~5000bp, ~700bp
DNA(CMV/MV9 C1, C2) 2.0 μL
10X buffer 1.5 μL
PstI 1.0 μL
NruI 1.0 μL
dH2O 9.5 μL
Total 15 μL --> 37°C/ 30 min.
Hover name
Expected Bands .
Hover name
Observed results.

Ladder


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