Haynes Lab:Notebook/Engineering PC-TFs/2014/07/09: Difference between revisions
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'''Restriction Digest''' | '''Restriction Digest''' | ||
{| {{table}} border="1" cellspacing=" | {| {{table}} border="1" cellspacing="5" | ||
|- valign="top" | |- valign="top" | ||
| bgcolor=#cfcfcf | Reagent | | bgcolor=#cfcfcf | Reagent | ||
| bgcolor=#cfcfcf | Volume | | bgcolor=#cfcfcf | Volume | ||
| rowspan=" | | rowspan="9" | <u>Expected:</u><br>BL01(W1:2-4), BL05(W1:5, W2:2-3) <br>Forward insertion: 710, 7010. <br>Reverse insertion: 3230, 4490. <br>No insertion: 710, 4490.<br> BL09 (W2:4-6) <br>Forward insertion: 710, 5921 <br>Reverse insertion: 2141, 4490 <br>No insertion: 710, 4490.<br> *Final well is empty CMV/MV9. | ||
| rowspan="7" | [[Image:W120140709 174255.jpg|400px|Today's gel]]<br>15 μL/lane; 1% agarose;<br> <u>Observed lengths:</u> W2: 7100, 700; W3: 7100, 5200, 2500; W4: *5200, 2500, 710; W5: 5000, 2500; W6: 5000, 2500, 710.<br> *Uncut plasmid.<br> [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | | rowspan="7" | [[Image:W120140709 174255.jpg|400px|Today's gel]]<br>15 μL/lane; 1% agarose;<br> <u>Observed lengths:</u> W2: 7100, 700; W3: 7100, 5200, 2500; W4: *5200, 2500, 710; W5: 5000, 2500; W6: 5000, 2500, 710.<br> *Uncut plasmid.<br> [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
| rowspan="7" | [[Image:W220140709 174229.jpg|400px|Today's gel]]<br>15 μL/lane; 1% agarose;<br> <u>Observed lengths:</u> W2: *7100, 700; W3: *6100, 2500, 710; W4: *7200, 2500, 710; W5: 5000, 4000; W6: *5500, 2500, 750.<br> *Uncut plasmid.<br> [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | | rowspan="7" | [[Image:W220140709 174229.jpg|400px|Today's gel]]<br>15 μL/lane; 1% agarose;<br> <u>Observed lengths:</u> W2: *7100, 700; W3: *6100, 2500, 710; W4: *7200, 2500, 710; W5: 5000, 4000; W6: *5500, 2500, 750.<br> *Uncut plasmid.<br> [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] |
Revision as of 13:47, 10 July 2014
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SummaryLiquid Culture Minipreps
Restriction Digest
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