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Summary
Ligation Digest and Gel Verification
Reagent
Volume
Expected:
BL09 Ligation Colonies 1-3[XbaI/NruI] (Well: 2-4)
Forward insertion: 710, 5921
Reverse insertion: 2141, 4490
No insertion: 710, 4490
CMV/MV9 with [XbaI/NruI] (Well: 5)
710, 4490
CMV/MV9 [XbaI](Well: 6)
5200
Dephos CMV/MV9 (Well: 7)
5200
15 μL/lane; 1% agarose;
Observed lengths:
W2(BL09Lig1): 5000, 7100, 2500;
W3(BL09Lig2): 5000, 7100, 2500;
W4(BL09Lig3): 5000, 7100, 2500;
W5(Empty CM9): 5000, 700;
W6(CM9XbaI): 10000, 20000, 5000.
W7(DephosCM9): 5000
Ladder
DNA(BL01x2, BL05x1, BL09x2, CMV/MV9x1)
3.0 μL
10X buffer
1.5 μL
XbaI
1.0 μL
NruI
1.0 μL
dH2 O
8.5 μL
Total
15 μL --> 37°C/ 30 min.