Haynes Lab:Notebook/Engineering PC-TFs/2014/10/11: Difference between revisions
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Diluted insert and vector down to 3nM using MW and known concentration.<br> | Diluted insert and vector down to 3nM using MW and known concentration.<br> | ||
<br> | <br> | ||
LCR Calculations | |||
* Ratio of Vector : Insert : Bridge oligo 1 : Bridge oligo 2 = 1 : 1 : ? : ? | |||
* X ng insert = (bp insert / bp vector) x 1 x 50 ng vector | |||
{| class="wikitable" width=400px align="left" | {| class="wikitable" width=400px align="left" | ||
| || BL01|| BL05 | | || BL01|| BL05 |
Revision as of 10:45, 20 October 2014
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Summary
Purified BL09 Negative Control Protein Extract
Digest
LCR LCR Calculations
Transformation
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