Haynes Lab:Notebook/Engineering PC-TFs/2014/10/13: Difference between revisions

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* Results:
* Results:


# BL09 1 = 181
# U2OS BL09 = 181
# BL09 2 (no transfection) = 25
# Mock Transfection = 25
# BL09 negative ctrl (no DNA) = 34
 




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# Standard 5 (8 μg) = 0.644
# Standard 5 (8 μg) = 0.644
# Standard 6 (16 μg) = 0.829
# Standard 6 (16 μg) = 0.829
# BL09 1 = 0.313
# U2OS BL09 = 0.313
# BL09 2 (no transfection) = 0.302
# Mock Transfection = 0.302
# BL09 negative = 0.048
 


Standard curve
Standard curve
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| Sample||Protein μg/μL||Protein*100 μL||Raw Fluor. (100 uL)||Fluor/ μg protein
| Sample||Protein μg/μL||Protein*100 μL||Raw Fluor. (100 uL)||Fluor/ μg protein
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|-
| BL09 1||0.765||76.50||181||2.365929
| U2OS BL09||0.765||76.50||181||2.365929
|-
| BL09 2||0.464||46.45||25||0.538235
|-
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| BL09 negative||-6.47||-647.54||34||-0.05251
| Mock Transfection||0.464||46.45||25||0.538235
|}
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Latest revision as of 00:25, 27 September 2017



 		Main project page
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Summary

  • RFP
  • Bradford

Plate reader measurements

RFP signal

  1. U2OS BL09 = 181
  2. Mock Transfection = 25


Bradford assay

  1. Standard 1 (0 μg) = 0.276
  2. Standard 2 (1 μg) = 0.234
  3. Standard 3 (2 μg) = 0.394
  4. Standard 4 (4 μg) = 0.443
  5. Standard 5 (8 μg) = 0.644
  6. Standard 6 (16 μg) = 0.829
  7. U2OS BL09 = 0.313
  8. Mock Transfection = 0.302


Standard curve


Final results:

Sample Protein μg/μL Protein*100 μL Raw Fluor. (100 uL) Fluor/ μg protein
U2OS BL09 0.765 76.50 181 2.365929
Mock Transfection 0.464 46.45 25 0.538235



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