Haynes Lab:Notebook/Engineering PC-TFs/2014/10/13: Difference between revisions
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<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
==Summary== | ==Summary== | ||
* | * RFP | ||
* Bradford | |||
'''Plate reader measurements''' | '''Plate reader measurements''' | ||
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# Standard 5 (8 μg) = 0.644 | # Standard 5 (8 μg) = 0.644 | ||
# Standard 6 (16 μg) = 0.829 | # Standard 6 (16 μg) = 0.829 | ||
# BL09 1 = 0. | # BL09 1 = 0.313 | ||
# BL09 2 (no transfection) = 0. | # BL09 2 (no transfection) = 0.302 | ||
# BL09 negative = 0. | # BL09 negative = 0.048 | ||
Standard curve | Standard curve | ||
[[Image: | [[Image:Bradford rfp.png|600px]] | ||
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| Sample||Protein μg/μL||Protein*100 μL||Raw Fluor. (100 uL)||Fluor/ μg protein | | Sample||Protein μg/μL||Protein*100 μL||Raw Fluor. (100 uL)||Fluor/ μg protein | ||
|- | |- | ||
| BL09 1|| | | BL09 1||0.765||76.50||181||2.365929 | ||
|- | |- | ||
| BL09 2|| | | BL09 2||0.464||46.45||25||0.538235 | ||
|- | |- | ||
| BL09 negative|| | | BL09 negative||-6.47||-647.54||34||-0.05251 | ||
|} | |} | ||
Revision as of 23:39, 13 October 2014
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Summary
Plate reader measurements RFP signal
Standard curve
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