Haynes Lab:Notebook/Engineering PC-TFs/2015/02/04: Difference between revisions
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(Autocreate 2015/02/04 Entry for Haynes_Lab:Notebook/Engineering_PC-TFs) |
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==Summary== | ==Summary== | ||
'''Digest and Gel Verification''' | |||
<br> | |||
{| {{table}} border="1" cellspacing="5" | |||
|- valign="top" | |||
| bgcolor=#cfcfcf | Reagent | |||
| bgcolor=#cfcfcf | Volume | |||
| rowspan="9" | | |||
<u><b>Expected:</b></u> | |||
<br> | |||
BL09: 55081.78Da <br><br> | |||
| rowspan="10" | [[Image:20150205 163642.jpg|400px|Today's gel]]<br> | |||
15 μL/lane; 1% agarose;<br> | |||
<u>Observed lengths:</u> <br> | |||
W1: Page Ruler Plus Prestained <br> | |||
W2: Purified BL09 <br> | |||
W3: Blank 11<br> | |||
W4: BL09: U2OS Cell Lysate<br> | |||
W5: Blank<br> | |||
W6: Precision Plus Protein Dual Color Standard<br> | |||
[http://openwetware.org/wiki/Image:Page ladder plus prestained.jpg Left Ladder] | |||
[http://openwetware.org/wiki/Image:Precision Plus Protein™ Dual Color Standards -161-0374.jpg] | |||
|- | |||
| 4X NuPAGE Dye|| 5.0 μL | |||
|- | |||
| 1M DTT || 1.0 μL | |||
|- | |||
| Protein Prep Buffer || 4.0 μL | |||
|- | |||
| Protein Sample|| 10.0 μL | |||
|- | |||
| dH<sub>2</sub>O || 0.0 μL | |||
|- | |||
| Total || 20 μL --> 100°C/ 5 min. | |||
|} | |||
<br> | |||
Observed: Possible PcTF band ~ 60kDa. PcTF reported to be larger in observation than theoretical size. | |||
Revision as of 10:11, 10 February 2015
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SummaryDigest and Gel Verification
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