Haynes Lab:Notebook/Engineering PC-TFs/2015/02/12: Difference between revisions
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==Summary== | ==Summary== | ||
'''Picking Colonies --> Liquid Cultures'''<br> | |||
No growth on negative control or BL09 lig2 plate. Will re-check concentration/purity of this plasmid. <br> | |||
~30 colonies for BL09 lig5 and BL01 LCR plate.<br><br> | |||
Picked three colonies from each plate and grew up in 4mL amp media. Incubated at 37°C and 250rpm overnight. | |||
Revision as of 11:02, 12 February 2015
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SummaryPicking Colonies --> Liquid Cultures Picked three colonies from each plate and grew up in 4mL amp media. Incubated at 37°C and 250rpm overnight.
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