Haynes Lab:Notebook/Engineering PC-TFs/2015/02/16: Difference between revisions
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==Summary== | ==Summary== | ||
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Added 1mL cold FACS to each sample 15mL tube (taken down from 2mL because cell pellet did not look giant).<br> | Added 1mL cold FACS to each sample 15mL tube (taken down from 2mL because cell pellet did not look giant).<br> | ||
Placed tubes on ice. <br> | Placed tubes on ice. <br> | ||
Added 1mL FACS to an empty tube for flow cytometry.<br><br> | Added 1mL FACS to an empty tube for flow cytometry.<br> | ||
Move everything outside of the TC Room.<br><br> | |||
'''Luciferase Assay'''<br> | '''Luciferase Assay'''<br> |
Latest revision as of 00:45, 27 September 2017
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SummaryHarvested GAL4EED-Luc Cells from 6-Well Plate Luciferase Assay Sample Preparation The current recommended program for reading luciferase activity on the Synergy H1 reader is:
Save and export data for analysis. Normalize plate reader values to cell count from flow cytometer (see below).
For data collection, hit the 'histogram' button. You will be making three graphs. The data is stored in the software so an retroactively change and delete the graph types. Cell Count Data within Gate for 20μL Sample from 2.17.2014 BL01 +Dox: 6630
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