Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/04/07: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
(fix raw html notebook nav) |
||
(One intermediate revision by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">DBN002 Gel Purification</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">DBN002 Gel Purification</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
Line 16: | Line 16: | ||
| align="center" style="background:#f0f0f0;"|'''stdev ng/µL''' | | align="center" style="background:#f0f0f0;"|'''stdev ng/µL''' | ||
|- | |- | ||
| DBN002||16. | | DBN002||16.84||1.261 | ||
|} | |} | ||
Latest revision as of 00:53, 27 September 2017
DBN002 Gel Purification | Main project page Previous entry Next entry | ||||||
OverviewI took the 55°C and 56°C fractions from the thermal gradient PCR of DBN002 with f2 primer (here) and ran them on a 1% agarose gel, then excised the 1444bp fragment and purified it using the GenElute gel extraction kit. DNA concentration (n=2):
Yield is low, but should be sufficient for Type IIS ligation.
|