Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/04/17: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 45: Line 45:
|}
|}


Digest for 15 minutes at 37°C.
Digest for 10 minutes at 37°C.




Revision as of 11:27, 17 April 2015

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Plasmid Miniprep of DBN001_pSB1A3 (repeat)

--Notes--

Once again, liquid culture density is very low. Suspect it's because there's no actual plasmid present.

--DNA Concentration--

Sample ID Conc. (ng/uL) St Dev (ng/uL) 260/280
DBN001_pSB1A3 colony 1 78.27 4.98 1.89
DBN001_pSB1A3 colony 2 59.95 4.37 2.14

--Restriction Digest--

Reagent Volume
DNA 600ng (7.7 - 10 µL)
10x FastDigest Buffer 3
EcoRI 1
PstI 1
Water 17.3 - 15
Total 30

Digest for 10 minutes at 37°C.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=Haynes_Lab:Notebook/HPK-CFP_insertion_into_Gal4EED/Luc_using_CRISPR/2015/04/17&oldid=880844"