Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2012/12/05: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==December 5, 2012== | ==December 5, 2012== | ||
'''Results from last 11/23/12 ligation''' | |||
'''Results from last | |||
* None of the colonies on any plates grew | * None of the colonies on any plates grew | ||
* Will retry cloning for each (H2B, LOV) by using the Gibson assembly method | * Will retry cloning for each (H2B, LOV) by using the Gibson assembly method | ||
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* Note: Primers add a XbaI site upstream and the SpeI site downstream | * Note: Primers add a XbaI site upstream and the SpeI site downstream | ||
* Final Gibson assembly products | * Final Gibson assembly products | ||
# pSB1A3 left end / H2B / LOV +His tag/ pSB1A3 right end | |||
# pSB1A3 left end / LOV / H2B +His tag/ pSB1A3 right end | |||
*For H2B-LOV construct | *For H2B-LOV construct | ||
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* Clean up PCR products using the Zymo Clean and Concentrator kit | * Clean up PCR products using the Zymo Clean and Concentrator kit | ||
* Be sure to elute using 15 μL dH<sub>2</sub>O | * Be sure to elute using 15 μL dH<sub>2</sub>O | ||
{| class="wikitable" border=1 cellpadding="5" cellspacing="0" | {| class="wikitable" border=1 cellpadding="5" cellspacing="0" | ||
|- | |- | ||
| | |Construct || Product || 260 || 280 || 260/280 || ng/μL | ||
|- | |- | ||
| | |H2B-LOV || H2B 1 || 0.061 || 0.033 || 1.863 || 60.545 | ||
|- | |- | ||
| | |H2B-LOV || H2B 2 || 0.056 || 0.03 || 1.857 || 56.22 | ||
|- | |- | ||
| | |H2B-LOV || LOV 1 || 0.063 || 0.033 || 1.903 || 62.673 | ||
|- | |- | ||
| | |H2B-LOV || LOV 2 || 0.06 || 0.04 || 1.85 || 59.844 | ||
|- | |- | ||
| | |LOV-H2B || H2B 1 || 0.072 || 0.033 || 1.807 || 72.381 | ||
|- | |- | ||
| | |LOV-H2B || H2B 2 || 0.06 || 0.036 || 1.799 || 59.925 | ||
| | |||
| H2B | |||
|- | |- | ||
| LOV - | |LOV-H2B || LOV 1 || 0.006 || 0.036 || 1.849 || 65.881 | ||
|- | |- | ||
| LOV - | |LOV-H2B || LOV 2 || 0.065 || 0.035 || 1.839 || 65.285 | ||
|} | |} | ||
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Latest revision as of 22:19, 26 September 2017
Project name | Main project page Previous entry Next entry | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
December 5, 2012Results from last 11/23/12 ligation
Performed table reactions twice: once for those used in H2B-LOV construct, once for those used in LOV-H2B construct
PCR program:
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