Haynes Lab:Notebook/Jan/2015/08/26: Difference between revisions
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==Making Stable Cell Lines from U2OS Cells Transfected with BD004/BD006== | ==Making Stable Cell Lines from U2OS Cells Transfected with BD004/BD006== | ||
* Passaged cells at a 1:10, 1:20, 1:40, dilution into 10 cm plates with fresh antibiotic-free growth medium 48 hours after transfection on 8/24/15. | * Passaged cells at a 1:10, 1:20, 1:40, dilution into 10 cm plates with fresh antibiotic-free growth medium 48 hours after transfection on 8/24/15. | ||
* Total number was 6 10-cm plates: 3 with BD004 and 3 with BD006 | * Total number was 6 10-cm plates: 3 with BD004 and 3 with BD006. | ||
* Also split U2OS cells. | * Also split U2OS cells for maintenance. | ||
Revision as of 16:53, 26 August 2015
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Making Stable Cell Lines from U2OS Cells Transfected with BD004/BD006
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