Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/01/06
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RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
SummaryTaking care of cell culture maintenance today, nothing special. Goals
Protocoltransformation of E. coli with plasmid DNA (quick method)
Materials
NotesObservations, measurements, placeholder info etc. go here
Results/ConclusionsPassaged SKNSH from PS-2 to PS-3. Some cells starting to show signs of changing morphology, clustering. DH5α plates (one plasmid, one negative control) are in the bacterial incubator in the main lab. To do: Transfect SKNSH cells in 6 well plate tomorrow, continue plasmid prep.
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