Haynes Lab:Notebook/Short Projects/2014/10/20: Difference between revisions
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Microscope Images: | Microscope Images: | ||
* Plate #1, Well 1A - Transfected with | * Plate #1, Well 1A - Transfected with 1160ng KAH60/pcVn | ||
[[Image:1A_DNA_phase_1.jpg|200px|Light microscope image of mammalian cells transfected with KAH60/pcVn. This plate was the control group for the transfection mini-experiment.]] | [[Image:1A_DNA_phase_1.jpg|200px|Light microscope image of mammalian cells transfected with KAH60/pcVn. This plate was the control group for the transfection mini-experiment.]] | ||
[[Image:1A_DNA_mCHERRY_1.jpg|200px|RFP signal of mammalian cells transfected with KAH60/pcVn. This plate was the control group for the transfection mini-experiment.]] | [[Image:1A_DNA_mCHERRY_1.jpg|200px|RFP signal of mammalian cells transfected with KAH60/pcVn. This plate was the control group for the transfection mini-experiment.]] | ||
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[[Image:1B_mock_mCHERRY_1.jpg|200px|RFP signal of mammalian cells that were mock transfected with DH2O. This plate was the control group for the transfection mini-experiment.]] | [[Image:1B_mock_mCHERRY_1.jpg|200px|RFP signal of mammalian cells that were mock transfected with DH2O. This plate was the control group for the transfection mini-experiment.]] | ||
* Plate #2, Well 2A - Transfected with | * Plate #2, Well 2A - Transfected with 1160ng KAH60/pcVn | ||
[[Image:2A_DNA_phase_1.jpg|200px|Light microscope image of mammalian cells transfected with KAH60/pcVn. This plate was centrifuged to increase plasmid uptake during transfection.]] | [[Image:2A_DNA_phase_1.jpg|200px|Light microscope image of mammalian cells transfected with KAH60/pcVn. This plate was centrifuged to increase plasmid uptake during transfection.]] | ||
[[Image:2A_DNA_mCHERRY_1.jpg|200px|RFP signal of mammalian cells transfected with KAH60/pcVn. This plate was centrifuged to increase plasmid uptake during transfection.]] | [[Image:2A_DNA_mCHERRY_1.jpg|200px|RFP signal of mammalian cells transfected with KAH60/pcVn. This plate was centrifuged to increase plasmid uptake during transfection.]] | ||
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[[Image:2B_mock_phase_1.jpg|200px|Light microscope image of mammalian cells that were mock transfected with DH2O. This plate was centrifuged to increase plasmid uptake during transfection.]] | [[Image:2B_mock_phase_1.jpg|200px|Light microscope image of mammalian cells that were mock transfected with DH2O. This plate was centrifuged to increase plasmid uptake during transfection.]] | ||
[[Image:2B_mock_mCHERRY_1.jpg|200px|RFP signal of mammalian cells that were mock transfected with DH2O. This plate was centrifuged to increase plasmid uptake during transfection.]] | [[Image:2B_mock_mCHERRY_1.jpg|200px|RFP signal of mammalian cells that were mock transfected with DH2O. This plate was centrifuged to increase plasmid uptake during transfection.]] | ||
* Because of the success of this transfection mini-experiment, a full-blown transfection will be completed with week. | * Because of the success of this transfection mini-experiment, a full-blown transfection will be completed with week. | ||
* Multiple minipreps (4 in total) will be completed in order to increase the final concentration of KAH60/pcVn plasmids | * Multiple minipreps (4 in total) will be completed in order to increase the final concentration of KAH60/pcVn plasmids | ||
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'''Bacteria Plated for Minipreps''' | |||
* New DH5α-Turbo cells were plated and incubated for KAH60/pcVn transduction | |||
Revision as of 15:12, 20 October 2014
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10/20/14 - Jonah Thomas
Note: Plate #1 was left as a control and Plate #2 was spun in a centrifuge (this was completed in hopes of increasing plasmid uptake)
Microscope Images:
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