10/20/14 - Jonah Thomas
- Reviewed the results of the transfection mini-experiment (see below for results)
- Plated new bacteria for KAH60/pcVn transduction
Transfection Mini-Experiment: Results
Note: Plate #1 was left as a control and Plate #2 was spun in a centrifuge (this was completed in hopes of increasing plasmid uptake)
- Plate #1 and Plate #2 both showed RFP signal
- The RFP signal from Plate #1 was stronger than the RFP signal from Plate #2
- It is hypothesized that the cells on Plate #2 died because of their increased sensitivity due to spinning; in the future, p/s-free medium should be used to prevent this from happening
Microscope Images:
- Plate #1, Well 1A - Transfected with 1160ng KAH60/pcVn
- Plate #1, Well 1B - Mock transfection with DH2O
- Plate #2, Well 2A - Transfected with 1160ng KAH60/pcVn
- Plate #2, Well 2B - Mock transfection with DH2O
- Because of the success of this transfection mini-experiment, a full-blown transfection will be completed with week.
- Multiple minipreps (4 in total) will be completed in order to increase the final concentration of KAH60/pcVn plasmids
Bacteria Plated for Minipreps
- New DH5α-Turbo cells were plated and incubated for KAH60/pcVn transduction
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