How To Make A MEGA-plate: Difference between revisions
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For a plate of 62cm on 82cm: | For a plate of 62cm on 82cm: | ||
* Polycarbonate sheet 62cm X 82cm | * Polycarbonate sheet 62cm X 82cm X 0.6cm (compeny,ID) | ||
* Plexiglas sheet 30cm X 60cm | * Plexiglas sheet 30cm X 60cm X 0.3cm (compeny,ID) | ||
* Dichloromethane | * Dichloromethane (compeny,ID) | ||
[[Image:Dichloromethane.jpg]] | [[Image:Dichloromethane.jpg]] | ||
* Syringe | * Syringe (compeny,ID) | ||
* Needle | * Needle (compeny,ID) | ||
* 9 X 2L Erlenmeyer flask | * 9 X 2L Erlenmeyer flask (compeny,ID) | ||
[[Image:Erlenmeyer flask.jpg]] | [[Image:Erlenmeyer flask.jpg]] | ||
* | * Agar (http://www.bd.com/,214010) | ||
==Procedure== | ==Procedure== | ||
| Line 26: | Line 26: | ||
## 4 pieces of 40cm X 2cm | ## 4 pieces of 40cm X 2cm | ||
## 2 pieces of 60cm X 2cm | ## 2 pieces of 60cm X 2cm | ||
# | |||
# Glue the pieces like the plan design | |||
## | ## | ||
## | ## | ||
Revision as of 06:40, 12 January 2017
Overview
Replace this sentence with a brief description of the protocol and its goal.
The goal is hoe to build a MEGA-plate.
Materials
For a plate of 62cm on 82cm:
- Polycarbonate sheet 62cm X 82cm X 0.6cm (compeny,ID)
- Plexiglas sheet 30cm X 60cm X 0.3cm (compeny,ID)
- Dichloromethane (compeny,ID)
- Syringe (compeny,ID)
- Needle (compeny,ID)
- 9 X 2L Erlenmeyer flask (compeny,ID)
- Agar (http://www.bd.com/,214010)
Procedure
- Plan the design of the plate.
- Use laser cutter to cut the plexiglas sheet to your design:
- 8 pieces of 40cm X 1.27cm
- 4 pieces of 40cm X 2cm
- 2 pieces of 60cm X 2cm
- Glue the pieces like the plan design
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
- List troubleshooting tips here.
- You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
- Anecdotal observations that might be of use to others can also be posted here.
Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.
References
Relevant papers and books
- Goldbeter, A and Koshland, DE (1981) - Proc Natl Acad Sci U S A 78(11) 6840-4 PMID 6947258
- Jacob, F and Monod, J J (1961) - Mol Biol 3(3) 318-56 PMID 13718526
- Ptashne, M (2004) Genetic Switch: Phage Lambda Revisited - Cold Spring Harbor Laboratory Press ISBN 0879697164
Contact
- Who has experience with this protocol?
or instead, discuss this protocol.
