HughesLab:Protocols: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
Erin Arant (talk | contribs) mNo edit summary |
||
| Line 3: | Line 3: | ||
== Molecular Biology == | == Molecular Biology == | ||
* Basic qPCR protocol using the ABI7900HT machine and Taqman probes: [[Image: | * Basic qPCR protocol using the ABI7900HT machine and Taqman probes: [[Image:RNA_prep_using_Trizol.docx]]<br> | ||
* Arcturus RiboAmp RNA amplification from fly brains: [[Image:RiboAmp1.docx]]<br> | * Arcturus RiboAmp RNA amplification from fly brains: [[Image:RiboAmp1.docx]]<br> | ||
* Basic RT reaction using ABI Archive cDNA kit: [[Image:RT_protocol.docx]]<br> | * Basic RT reaction using ABI Archive cDNA kit: [[Image:RT_protocol.docx]]<br> | ||
Revision as of 14:54, 7 February 2017
Home Lab Members Research JTK_Cycle MuscleDB Publications Protocols Academic Analytics Open Positions Contact
Molecular Biology
- Basic qPCR protocol using the ABI7900HT machine and Taqman probes: File:RNA prep using Trizol.docx
- Arcturus RiboAmp RNA amplification from fly brains: File:RiboAmp1.docx
- Basic RT reaction using ABI Archive cDNA kit: File:RT protocol.docx
- RNA prep using Trizol and Qiagen RNeasy columns: File:RNA prep using Trizol.docx
Next-generation sequencing
- Pilot protocol for PCR amplifying fly RNA editing sites: File:Editingseq pilot.docx
Fly Behavior and Genetics
- Drosophila virgin collection: File:Drosophila virgin collection.docx
- Vials for DAM analysis: File:Fly food for DAM analysis.docx
Analytics
- Human temperature record analysis: File:Human Temp Analysis.docx
- RUM (RNA-Seq Unified Mapper) guide: File:RUM guide.pdf
- Basic linux commands: File:Linux commands.pdf
Hughes Lab
Pulmonary and Critical Care Medicine
Washington University School of Medicine
