HughesLab:Protocols

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Molecular Biology

• Basic qPCR protocol using the ABI7900HT machine and Taqman probes: Image:QPCR protocol.docx
  
• Arcturus RiboAmp RNA amplification from fly brains: Image:RiboAmp1.docx
  
• Basic RT reaction using ABI Archive cDNA kit: Image:RT protocol.docx
  
• RNA prep using Trizol and Qiagen RNeasy columns: Image:RNA prep using Trizol.docx

Next-generation sequencing

• Pilot protocol for PCR amplifying fly RNA editing sites: Image:Editingseq pilot.docx
  

Fly Behavior and Genetics

• Drosophila virgin collection: Image:Drosophila virgin collection.docx
• Vials for DAM analysis: Image:Fly food for DAM analysis.docx

Analytics

• Human temperature record analysis: Image:Human Temp Analysis.docx

Hughes Lab
Department of Biology
University of Missouri, St. Louis
(Starting in August, 2013)