HughesLab:Protocols: Difference between revisions
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* ''Drosophila'' virgin collection: [[Image:Drosophila_virgin_collection.docx]] | * ''Drosophila'' virgin collection: [[Image:Drosophila_virgin_collection.docx]] | ||
* Vials for DAM analysis: [[Image:Fly_food_for_DAM_analysis.docx]] | * Vials for DAM analysis: [[Image:Fly_food_for_DAM_analysis.docx]] | ||
* Fly food protocol: [[Image:Fly_food.docx]] | |||
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Revision as of 10:00, 5 July 2017
Home Lab Members Research JTK_Cycle MuscleDB Publications Protocols Academic Analytics Open Positions Contact
Molecular Biology
- Basic qPCR protocol using the ABI7900HT machine and Taqman probes: File:RNA prep using Trizol.docx
- Arcturus RiboAmp RNA amplification from fly brains: File:RiboAmp1.docx
- Basic RT reaction using ABI Archive cDNA kit: File:RT protocol.docx
- RNA prep using Trizol and Qiagen RNeasy columns: File:RNA prep using Trizol.docx
Next-generation sequencing
- Pilot protocol for PCR amplifying fly RNA editing sites: File:Editingseq pilot.docx
Fly Behavior and Genetics
- Drosophila virgin collection: File:Drosophila virgin collection.docx
- Vials for DAM analysis: File:Fly food for DAM analysis.docx
- Fly food protocol: File:Fly food.docx
Analytics
- Human temperature record analysis: File:Human Temp Analysis.docx
- RUM (RNA-Seq Unified Mapper) guide: File:RUM guide.pdf
- Basic linux commands: File:Linux commands.pdf
Hughes Lab
Pulmonary and Critical Care Medicine
Washington University School of Medicine
