IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/30: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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* Ligation:
* Ligation:
: TesA+RBS insert with LacI+backbone
: TesA+RBS insert with LacI+backbone
:
{| {{table}} border="1" align=center <!-- Digestion table -->
|- valign="top"
| bgcolor=#cfcfcf | Reagent
| bgcolor=#cfcfcf | 1 (μL)
| bgcolor=#cfcfcf | 2 (μL)
| bgcolor=#cfcfcf | Control (μL)
|-
|| TesA+RBS || 2.0 || 2.0 || 0.0
|-
|| LacI || 3.0 || 4.0 || 3.0
|-
|| Ligation Buffer || 1.0 || 1.0 || 1.0
|-
|| Ligase || 1.0 || 1.0 || 1.0
|-
|| H<sub>2</sub>O || 3.0 || 2.0 || 5.0
|-


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Latest revision as of 00:09, 27 September 2017

Project name Main project page
Previous entry      Next entry

Wednesday 7/30

  • Mini Prep:
Second attempt
Isolated stocks of TesA+RBS and LacI plasmids
  • Gel Extraction:
TesA+RBS cut at X and P sites
LacI cut at S and P sites
260/280 ratio for LacI is shady, ~1.002
  • Ligation:
TesA+RBS insert with LacI+backbone
Reagent 1 (μL) 2 (μL) Control (μL)
TesA+RBS 2.0 2.0 0.0
LacI 3.0 4.0 3.0
Ligation Buffer 1.0 1.0 1.0
Ligase 1.0 1.0 1.0
H2O 3.0 2.0 5.0
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