Tuesday 7/29
- Miniprepped the lacI and the TesA+ RBS. There was a lot of cells with both.
- Did gel of both, and those did not turn out well. The LacI was streaked due to contamination and degradation and the the TesA + RBS was too close to the well so there was probably error in either the miniprep or the digestions of them. TesA+ RBS was digested at the x and p sites while the lacI was digested at the s and p sites.
Had to restart those. Did the seeds of both, and re-streaked the TesA +RBS from plate 4, colony 3.
Reagent
|
Volume
|
Lanes+Length: 1. 1 kb Ladder 3. LacI = 2000 5. TesA+RBS = 650
|
|
DNA (LacI plasmid) |
10.0 μL
|
10X buffer |
2.0
|
PstI |
1.0
|
SpeI |
1.0
|
dH2O |
6.0
|
|
20.0 μL --> 37°C/ ~1 Hr.
|
|