IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/30: Difference between revisions
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(Autocreate 2014/07/30 Entry for IGEM:Arizona_State/2014/Notebook/asu_igem_2014) |
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== | ==Wednesday 7/30== | ||
* | * Mini Prep: | ||
: Second attempt | |||
: Isolated stocks of TesA+RBS and LacI plasmids | |||
* Gel Extraction: | |||
: TesA+RBS cut at X and P sites | |||
: LacI cut at S and P sites | |||
: 260/280 ratio for LacI is shady, ~1.002 | |||
* Ligation: | |||
: TesA+RBS insert with LacI+backbone | |||
{| {{table}} border="1" align=center <!-- Digestion table --> | |||
|- valign="top" | |||
| bgcolor=#cfcfcf | Reagent | |||
| bgcolor=#cfcfcf | 1 (μL) | |||
| bgcolor=#cfcfcf | 2 (μL) | |||
| bgcolor=#cfcfcf | Control (μL) | |||
|- | |||
|| TesA+RBS || 2.0 || 2.0 || 0.0 | |||
|- | |||
|| LacI || 3.0 || 4.0 || 3.0 | |||
|- | |||
|| Ligation Buffer || 1.0 || 1.0 || 1.0 | |||
|- | |||
|| Ligase || 1.0 || 1.0 || 1.0 | |||
|- | |||
|| H<sub>2</sub>O || 3.0 || 2.0 || 5.0 | |||
|- | |||
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Revision as of 14:12, 1 August 2014
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Wednesday 7/30
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