IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/08/13: Difference between revisions
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== | ==8/13== | ||
<u>Competent Cell Check</u> | |||
*Ligation results suggest a problem with competent cells. | |||
**Transformed 3 μL of TetR promoter into 3 tubes of 50 μL of comp. cells taken from 2 different tubes. | |||
<u>Ligation</u> | <u>Ligation</u> | ||
*Ligation from 8/12 did not show any growth on any plates, suggesting competent cells may be bad. | |||
*Started a new ligation to save until competent cells can be checked. | |||
TesA+RBS insert with LacI+backbone | TesA+RBS insert with LacI+backbone | ||
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|| H<sub>2</sub>O || 4.0 || 6.0 | || H<sub>2</sub>O || 4.0 || 6.0 | ||
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<u>Ethanol</u> | |||
*Started seed of PACYC from plate started yesterday. | |||
*Started seed of BL21 in preparation to make competent cells. | |||
Revision as of 11:43, 13 August 2014
 Project name
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8/13Competent Cell Check
Ligation
TesA+RBS insert with LacI+backbone
Ethanol
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