IGEM:Berkeley/2010: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
No edit summary
Line 94: Line 94:
[[Template:SBB-Protocols_Enz1 | EcoRI/BamHI Digest of Wobble Products]]<br>
[[Template:SBB-Protocols_Enz1 | EcoRI/BamHI Digest of Wobble Products]]<br>
[[Template:SBB-Protocols_Enz2 | EcoRI/BamHI Digest of PCR Products]]<br>
[[Template:SBB-Protocols_Enz2 | EcoRI/BamHI Digest of PCR Products]]<br>
[[Manual 2ab Assembly Digest]] <br>
[[Template:SBB-Protocols_Enz3 | BglII Digest of EIPCR Products]]<br>
[[Template:SBB-Protocols_Enz3 | BglII Digest of EIPCR Products]]<br>
[[Template:SBB-Protocols_Enz4 | Ligation of EcoRI/BamHI digests]]<br>
[[Template:SBB-Protocols_Enz4 | Ligation of EcoRI/BamHI digests]]<br>

Revision as of 12:09, 11 June 2010

Project Name

Project Summary

<html> <img src="http://openwetware.org/images/b/b9/Icon_board.png" alt="Resources"> </html>The Team

Undergrads

Amy Kristofferson
Conor McClune
Tahoura Samad
Daniela
Christoph Neyer

Graduate Students
Tim Hsiau

Postdocs

Insert names here

Advisors
Chris Anderson
Terry Johnson


<html> <img src="http://openwetware.org/images/e/e2/Icon_info.png" alt="News" border="0"> </html>Tools

Lab calendar

Lab Notebooks

Group
Amy
Conor
Daniela
Tahoura
Christoph
Tim
The Details

Oligonucleotides
Gel Pictures
Sequencing
Construction Files
-80 Stocks
Assembly Trees
New Composite Parts



Procedures

Polymerase Reactions
Wobble Reaction
Cloning by PCR
SOEing PCR
PCA Gene Synthesis
QuikChange
Colony PCR

Cleanup Reactions
Regular Zymo Cleanup
Small-Frag Zymo Cleanup
Zymo Gel Purification

Digestions Reactions
EcoRI/BamHI Digest of Wobble Products
EcoRI/BamHI Digest of PCR Products
Manual 2ab Assembly Digest
BglII Digest of EIPCR Products
Ligation of EcoRI/BamHI digests
Ligation of EIPCR digests
Analytical digests (Mapping)

Bacteria Manipulations
Transformation by heat-shock
Picking of colonies
Miniprep purification of DNA
Macherey-Nagel Miniprep
-80 Glycerol Stocks
Making Competent Cells

Other
LR Gateway Transfers
EcoRI/BamHI Part Transfers
What do I do with my sequencing data?
Small Scale Competent Cell Transformation
ELISA
2ab Assembly


Subgroup Notebooks



<html> <img src="http://openwetware.org/images/b/b9/Icon_board.png" alt="Resources"> </html>Project


Useful Links


Subgroup Strategies, Overview

Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:Berkeley/2010&oldid=422446"